Monovalent lectin as a novel tool for the resolution of microheterogeneity in glycoproteins

Surolia, Avadhesha ; Appukuttan, P. S. ; Pain, Debkumar ; Bachhawat, B. K. (1980) Monovalent lectin as a novel tool for the resolution of microheterogeneity in glycoproteins Analytical Biochemistry, 105 (2). pp. 436-440. ISSN 0003-2697

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...

Related URL: http://dx.doi.org/10.1016/0003-2697(80)90563-1

Abstract

Microheterogeneous populations of the glycoprotein fetuin were found to bind molecules of the galactose-binding lectin ricin in proportion to the degree of desialation of the former and the number of galactose moieties exposed as a result. Monitoring the difference in molecular weight of the fetuin-ricin complexes formed from radioiodinated ricin, native fetuin could be resolved into three broad heterogeneous groups.

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