Homogeneous enzyme immunoassay of estriol in picogram amounts

Ghosh, Mridul ; Dhar, Tarun K. ; Ali, Esahak ; Bachhawat, B. K. (1983) Homogeneous enzyme immunoassay of estriol in picogram amounts Clinica Chimica Acta, 128 (2-3). pp. 223-231. ISSN 0009-8981

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000989...

Related URL: http://dx.doi.org/10.1016/0009-8981(83)90322-4


A homogeneous enzyme immunoassay for estriol has been described using estriol-glucose-6-phosphate dehydrogenase (E3G6PD) as enzyme conjugate. Antisera for estriol were raised by immunising rabbits with two different immunogens, estriol-6-(O-carboxymethyl oxime) bovine serum albumin (E36CMOBSA) and estriol-3-carboxymethyl ether bovine serum albumin (E33CMEBSA). A new method for preparation of estriol 3-O-carboxy methyl ether in high yield (90%) has also been described. Addition of anti-estriol antibodies to E3-G6PD conjugate resulted in inhibition of enzyme activity. In the presence of free estriol, the antibody induced inhibition of enzyme activity was reduced in a concentration dependent manner. The use of the heterologous combination between immunogen and enzyme conjugate i.e. using anti-E33CMEBSA and E36CMOG6PD improves the sensitivity at the expense of specificity, the cross-reaction with other estrogenic hormone being 10-15%.

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