Abnormal erythrocyte membrane phospholipid organisation in chronic myeloid leukaemia

Kumar, A. ; Daniel, S. ; Agarwal, S. S. ; Gupta, C. M. (1987) Abnormal erythrocyte membrane phospholipid organisation in chronic myeloid leukaemia Journal of Biosciences, 11 (1-4). pp. 543-548. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/11/543-548.pdf

Related URL: http://dx.doi.org/10.1007/BF02704703


The membrane phospholipid organisation in the red cells of humans suffering from chronic myeloid leukaemia has been analysed using the amino-group labelling reagent trinitrobenzenesulphonic acid and the fluid-sensing fluorophore, Merocyanine 540. Unlike the normal human erythrocytes, trinitrobenzenesulphonic acid in intact chronic myeloid leukaemia erythrocytes modified about 30% phosphatidylserine, under controlled conditions. Also, the chronic myeloid laukaemia red cells, but not the normal cells, were found to bind the fluorescent dye Merocyanine 540. These results demonstrate that loss of the transmembrane phospholipid asymmetry in chronic myeloid leukaemia erythrocytes is accompanied by an enhancement in the outer surface fluidity and, therefore, suggest that the red cells membrane phase-state asymmetry originates probably from the asymmetric arrangements of phospholipids across the membrane bilayer.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Phospholipid Asymmetry; Erythrocytes; Phase-state Asymmetry; Chronic Myeloid Leukaemia
ID Code:224
Deposited On:17 Sep 2010 09:18
Last Modified:16 May 2016 11:30

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