Mohanty, Samarendra K. ; Sharma, Mrinalini ; Gupta, Pradeep K. (2003) Laser-assisted microinjection into targeted animal cells Biotechnology Letters, 25 (11). pp. 895-899. ISSN 0141-5492
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Official URL: http://www.springerlink.com/content/uj64k241138036...
Related URL: http://dx.doi.org/10.1023/A:1024038609045
Abstract
A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer-Verlag. |
| Keywords: | Microinjection; Optoporation; Photodynamic Treatment; Transfection |
| ID Code: | 22321 |
| Deposited On: | 23 Nov 2010 08:57 |
| Last Modified: | 02 Jun 2011 07:05 |
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