Restriction enzyme digestion of heterochromatin in Drosophila nasuta

Tiwari, P. K. ; Lakhotia, S. C. (1991) Restriction enzyme digestion of heterochromatin in Drosophila nasuta Journal of Biosciences, 16 (4). pp. 187-197. ISSN 0250-5991

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In situ digestion of metaphase and polytene chromosomes and of interphase nuclei in different cell types of Drosophila nasuta with restriction enzymes revealed that enzymes like AluI, EcoRI, HaeIII, Sau3a and SinI did not affect Giemsa-stainability of heterochromatin while that of euchromatin was significantly reduced; TaqI and SalI digested both heterochromatin and euchromatin in mitotic chromosomes. Digestion of genomic DNA with AluI, EcoRI, HaeIII, Sau3a and KpnI left a 23 kb DNA band undigested in agarose gels while with TaqI, no such undigested band was seen. The AluI resistant 23 kb DNA hybridized insitu specifically with the heterochromatic chromocentre. It appears that the digestibility of heterochromatin region in genome ofDrosophila nasuta with the tested restriction enzymes is dependent on the availability of their recognition sites.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Drosophila; Heterochromatin; Restriction Enzyme Digestion
ID Code:22140
Deposited On:23 Nov 2010 08:15
Last Modified:17 May 2016 06:14

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