Separation of polypeptide chains of ricin and the interaction of the A chain with cibacron blue F3GA

Appukuttan, P. S. ; Bachhawat, B. K. (1979) Separation of polypeptide chains of ricin and the interaction of the A chain with cibacron blue F3GA Biochimica et Biophysica Acta (BBA) - Protein Structure, 580 (1). pp. 10-14. ISSN 0005-2795

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...

Related URL: http://dx.doi.org/10.1016/0005-2795(79)90192-2

Abstract

From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose. The presence of a nucleotide-binding domain on the toxic chain A could be demonstrated from its interaction with the blue dye Cibacron Blue F3GA.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Ricin Polypeptide Chain; Cibacron Blue F3GA; Galactomannan; Nucleotide-binding Domain
ID Code:2206
Deposited On:08 Oct 2010 07:33
Last Modified:12 May 2011 12:00

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