Enzymatic desulphation of cerebroside-3-sulphate by chicken brain arylsulphatase A

Abstract

In earlier work from this laboratory it was shown that arylsulphatase of chicken brain resembles arylsulphatase A of other animal species in several of its properties but exhibits certain characteristics similar to that of arylsulphatase B (Farooqui and Bachhawat, 1971). Recently the arylsulphatase A of chicken brain was purified and it was demonstrated that the purified enzyme could desulphate cerebroside-3-sulphate also (Farooqui and Bachhawat, 1972). In the present report we have made a study of the kinetic properties of this unique arylsulphatase A purified from chicken brain using p-nitrocatechol sulphate and cerebroside-3-sulphate as substrates.