Purification of the enhancing factor from mouse intestines

Mulherkar, Rita ; Saraf, Anjali ; Wagle, Archana ; Deo, M. G. (1986) Purification of the enhancing factor from mouse intestines FEBS Letters, 207 (1). pp. 142-144. ISSN 0014-5793

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...

Related URL: http://dx.doi.org/10.1016/0014-5793(86)80028-X


A unique polypeptide, called enhancing factor (EF), which enhances the binding of labeled epidermal growth factor (EGF) to cells, has been isolated. It has been purified to homogeneity from the acid-soluble proteins of mouse intestines. Earlier, EF was partially purified by two cycles of gel-permeation chromatography on Bio-Gel columns. We now report the final purification of EF on high-performance liquid chromatography (HPLC), using a reverse-phase column (μBondapak C18). The purity of the protein was confirmed when a single peak was obtained in HPLC. Also, a single protein band was obtained in SDS-PAGE. Purified EF has the same properties in vitro as those reported earlier for partially purified EF.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:Growth Factor; HPLC; Receptor Modulation
ID Code:21742
Deposited On:22 Nov 2010 10:54
Last Modified:17 May 2016 05:55

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