Studies on the mechanism of follitropin action at the cellular level

Vidyashankar, N. ; Moudgal, N. R. (1981) Studies on the mechanism of follitropin action at the cellular level Archives of Biochemistry and Biophysics, 209 (1). pp. 241-248. ISSN 0003-9861

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With a view toward understanding better the mechanism of action of follitropin, an attempt was made using granulosa cells obtained from intact immature estrogenized rats to study in short-term incubations the effect of highly purified ovine follitropin on the binding of the hormone to the cells and the associated aromatase response. A modified radioimmunoassay procedure has been used to monitor unlabeled physiologically fully active follitropin bound to the cell. A linear relationship between the actual amount of hormone bound to the cells and the estradiol produced in vitro has been established. The amount of ovine follitropin bound that can elicit a half-maximal response in estrogen production was calculated to be 400 pg. The number of follitropin binding sites per cell was 375 and the Kd of binding was 3.03 × 10-10 M . By the addition of ovine follitropin antiserum at different time points of a 4-h incubation period, a continual need for follitropin support for estradiol production has been established.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:21724
Deposited On:22 Nov 2010 10:57
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