Use of epoxysepharose for protein immobilisation

Murthy, G. S. ; Moudgal, N. R. (1986) Use of epoxysepharose for protein immobilisation Journal of Biosciences, 10 (3). pp. 351-358. ISSN 0250-5991

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Epoxy Sepharose, an activated affinity matrix which has been used for immobilisation of carbohydrates has been tried for immobilisation of proteins. Under normal conditions of coupling at neutral or alkaline pH proteins do not couple to epoxy Sepharose. However, a very high salt concentration during coupling allows the binding of proteins to epoxy Sepharose at a pH as low as 8.5. Increasing ionic strength and/or pH facilitates the binding. The bioactivity of the proteins is not destroyed by the immobilisation. This matrix, unlike cyanogen bromide-Sepharose, retains its ability to bind albumin by 80-90% even after 60 days of storage in aqueous suspension at 4°C. Its capacity to bind proteins is comparable to that of cyanogen bromide-Sepharose.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Epoxy Sepharose; Affinity Matrix; Protein Immobilisation
ID Code:21658
Deposited On:22 Nov 2010 11:06
Last Modified:17 May 2016 05:50

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