Ghosh, Mridul ; Surolia, A. ; Bachhawat, B. K. (1983) A simple and rapid method for quantification of hapten specific antibodies Journal of Immunological Methods, 57 (1-3). pp. 295-300. ISSN 0022-1759
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002217...
Related URL: http://dx.doi.org/10.1016/0022-1759(83)90090-X
Abstract
A sensitive and simple method for quantification of antibodies against small molecules is described using DNP-lysozyme as the enzyme conjugate. The anti-DNP antiserum was raised against DNP-bovin serum albumin conjugate. Anti-DNP antibody or its monovalent fragment (Fab) reduced the enzyme activity of DNP-lysozyme conjugate in a concentration-dependent manner. The inhibition of enzyme activity is a specific measure of the antibody and Fab content of the sample. The specificity of the reaction was assessed by reduction of antibody-induced inhibition by DNP-lysine. The ability of DNP-lysine to reduce the antibody-induced inhibition of DNP-lysozyme activity also makes possible a sensitive assay for DNP-lysine.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Anti-hapten Assay; DNP-lysine; Anti-DNP Antibody Assay |
| ID Code: | 2164 |
| Deposited On: | 08 Oct 2010 07:41 |
| Last Modified: | 14 Jun 2011 05:42 |
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