Specific binding of luteinizing hormone to leydig tumor cells

Moudgal, N. R. ; Moyle, W. R. ; Greep, R. O. (1971) Specific binding of luteinizing hormone to leydig tumor cells Journal of Biological Chemistry, 246 (16). pp. 4983-4986. ISSN 0021-9258

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Official URL: http://www.jbc.org/content/246/16/4983.short


A radioimmunoassay was used to detect luteinizing hormone (LH) bound to washed Leydig tumor cells. Tumor cell suspensions were incubated with LH at 37° and washed repeatedly by centrifugation with isotonic 0.9% NaCl solution. The tumor cells contained large quantities of LH even after they were washed sufficiently to produce a 106-fold dilution of unbound LH. Six washings (106-fold dilution) were no more effective in removing LH from the cells than three washings (103-fold dilution). Binding was not influenced by the temperature at which the cells were washed. The extent of LH binding was related to the number of cells, with approximately 5300 ± 960 molecules of LH bound per cell. LH binding was also proportional to the same concentrations of LH which produced a steroidogenic dose response curve. The binding constant of 1.5 × 10-8 m was considered to be higher than that expected for nontumorous tissues. Tumor cells bound more LH than did erythrocytes or thymocytes under the same conditions.

Item Type:Article
Source:Copyright of this article belongs to American Society for Biochemistry and Molecular Biology.
ID Code:21582
Deposited On:22 Nov 2010 06:44
Last Modified:17 May 2016 05:46

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