Immunization of male bonnet monkeys (M. radiata) with a recombinant FSH receptor preparation affects testicular function and fertility

Moudgal, N. R. ; Sairam, M. R. ; Krishnamurthy, H. N. ; Sridhar, Surekha ; Krishnamurthy, H. ; Khan, H. (1997) Immunization of male bonnet monkeys (M. radiata) with a recombinant FSH receptor preparation affects testicular function and fertility Endocrinology, 138 (7). pp. 3065-3068. ISSN 0013-7227

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Official URL: http://endo.endojournals.org/cgi/content/abstract/...

Related URL: http://dx.doi.org/10.1210/en.138.7.3065

Abstract

Immunization of proven fertile adult male monkeys (n = 3) with a recombinant FSH receptor protein preparation (oFSHR-P) (representing amino acids 1-134 of the extracellular domain of the receptor Mr ~15KDa) resulted in production of receptor blocking antibodies. The ability of the antibody to bind a particulate FSH receptor preparation and receptors in intact granulosa cells was markedly (by 30-80%) inhibited by FSH. Serum T levels and LH receptor function following immunization remained unchanged. The immunized monkeys showed a 50% reduction (p < 0.001) in transformation of spermatogonia (2C) to primary spermatocytes (4C) as determined by flow cytometry and the 4C:2C ratio showed a correlative change (R 0.81, p < 0.0007) with reduction in fertility index (sperm counts X motility score). Breeding studies indicated that monkeys became infertile between 242-368 days of immunization when the fertility index was in the range of 123 ± 76 to 354 ± 42 (compared to a value of 1602 ± 384 on day 0). As the effects observed are near identical to that seen following immunization with FSH it is suggestive that oFSHR-P can substitute for FSH in the development of a contraceptive vaccine.

Item Type:Article
Source:Copyright of this article belongs to Endocrine Society.
ID Code:21564
Deposited On:22 Nov 2010 11:37
Last Modified:17 May 2016 05:45

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