Tachykinin family genes and their receptors are differentially expressed in the hypothyroid ovary and pituitary

Ghosha, Pamela ; Saha, samir ; bhattacharya, Sabarna ; Bhattacharya, Samir ; Mukherjee, Satinath ; Roya, Sib (2007) Tachykinin family genes and their receptors are differentially expressed in the hypothyroid ovary and pituitary Cellular Physiology and Biochemistry, 20 (5). pp. 357-368. ISSN 1015-8987

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Related URL: http://dx.doi.org/10.1159/000107521


Plasma tachykinin levels are known to be altered with sexual acyclicity and loss of reproductive function. Ovulatory dysfunction, as seen in postmenopausal women, is also often encountered in hypothyroid patients. To know the involvement of different tachykinin genes in hypothyroidism-associated reproductive disorders, we performed DD-PCR with the pituitary RNA of control and hypothyroid rats to see the differentially expressed gene profile. Subsequently, we selected a few clones, tachykinin being one of them. Since its expression was up regulated in hypothyroidism as it does in the sexually acyclic females, we wanted to correlate these two phenomena with hypothyroidism associated reproductive disorders. We observed differential expression of tac2 along with other tk genes and their receptors in rat pituitary and ovary, which suggests that hypothyroidism affects the expression of these genes in these tissues. The experiments were repeated in ovarian tissue obtained at surgery from hypothyroid human patients, which showed similar expression pattern of TAC3 (equivalent to rat tac2) and their receptors as in rat ovary. Significant reduction of tac2 expression in reproductively less active rat ovary suggests the association of tac2 with reproductive senescence. Our results suggest that decline in reproductive function in hypothyroidism is associated with altered expression level of tac2 and its receptors. Further investigation in this area could elucidate the possible mechanism of tachykinins' involvement in loss of sexual cyclicity and other reproductive disorders associated with hypothyroidism.

Item Type:Article
Source:Copyright of this article belongs to Karger Publisher.
Keywords:Tachykinin; Pituitary; Ovary; Granulosa Cell; Hypothyroid; Thyroid Hormone; Reproductive Disorder
ID Code:21554
Deposited On:22 Nov 2010 06:47
Last Modified:17 May 2016 05:45

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