A Lupinoside prevented fatty acid induced inhibition of insulin sensitivity in 3T3 L1 adipocytes

Dey, Debleena ; Pal, Bikash C. ; Biswas, Tanushree ; Roy, Sib Sankar ; Bandyopadhyay, Arun ; Mandal, Swapan Kumar ; Giri, Bir Bhanu ; Bhattacharya, Samir (2007) A Lupinoside prevented fatty acid induced inhibition of insulin sensitivity in 3T3 L1 adipocytes Molecular and Cellular Biochemistry, 300 (1-2). pp. 149-157. ISSN 0300-8177

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Official URL: http://www.springerlink.com/index/v26j623qm8159645...

Related URL: http://dx.doi.org/10.1007/s11010-006-9378-1


The decrease in insulin sensitivity to target tissues or insulin resistance leads to type 2 diabetes mellitus, an insidious disease threatening global health. Numerous evidences made free fatty acids (FFAs) responsible for insulin resistance and type 2 diabetes. We demonstrate here that the damage of insulin acitivity by a free fatty acid, palmitate could be prevented by a lupinoside. An incubation of 3T3 L1 adipocytes with a FFA i.e. palmitate inhibited insulin stimulated uptake of 3H-2 deoxyglucose (2 DOG) significantly. Addition of a lupinoside purified from Pueraria tuberosa, lupinoside PA4 (LPA4) strongly prevented this inhibition. We then examined insulin signaling pathway where palmitate significantly inhibited insulin stimulated phosphorylation of Insulin receptor tyrosine kinase, IRS 1and PI3 kinase, PDK1 and Akt/PKB. LPA4 rescued this inhibition of signaling molecule by palmitate. Insulin mediated translocation of Glut4, the glucose transporter in insulin target cells, was effectively blocked by palmitate while, LPA4 waived this block. Administration of LPA4 to nutritionally induced diabetic rats significantly reduced the increase in plasma glucose. All these indicate LPA4 to be a potentially therapeutic agent for insulin resistance and type 2 diabetes.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Diabetes Type 2; Insulin Signal; Free Fatty Acids; Glut 4 Translocation; Insulin Resistance; Lupinoside
ID Code:21546
Deposited On:22 Nov 2010 06:48
Last Modified:21 Jan 2011 03:59

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