Inhibition of monkey liver serine hydroxymethyltransferase by Cibacron Blue 3G-A

Ramesh, K. S. ; Appaji Rao, N. (1980) Inhibition of monkey liver serine hydroxymethyltransferase by Cibacron Blue 3G-A Biochemical Journal, 187 . pp. 249-252. ISSN 0264-6021

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Official URL: http://www.biochemj.org/bj/187/bj1870249.htm

Abstract

Cibacron Blue 3G-A inhibited monkey liver serine hydroxymethyltransferase competitively with respect to tetrahydrofolate and non-competitively with respect to L-serine. NADH, a positive heterotropic effector, failed to protect the enzyme against inhibition by the dye and was unable to desorb the enzyme from Blue Sepharose CL-6B gel matrix. The binding of the dye to the free enzyme was confirmed by changes in the dye absorption spectrum. The results indicate that the dye probably binds at the tetrahydrofolate-binding domain of the enzyme, rather that at the 'dinucleotide fold'.

Item Type:Article
Source:Copyright of this article belongs to Portland Press Limited.
ID Code:21242
Deposited On:20 Nov 2010 13:16
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