Purification, physicochemical and regulatory properties of serine hydroxymethyltransferase from sheep liver

Manohar, R. ; Ramesh, K. S. ; Appaji Rao, N. (1982) Purification, physicochemical and regulatory properties of serine hydroxymethyltransferase from sheep liver Journal of Biosciences, 4 (1). pp. 31-50. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/4/31-50.pdf

Related URL: http://dx.doi.org/10.1007/BF02702579


Serine hydroxymethyltransferase (EC was purified from the cytosolic fraction of sheep liver by ammonium sulphate fractionation, CM-Sephadex chromatography, gel filtration using Ultrogel ACA 34 and Blue Sepharose affinity chromatography. The homogeneity of the enzyme was rigorously established by Polyacrylamide gel and sodium dodecyl sulphate-polyacrylamide gel electrophoresis, isoelectrofocusing, ultracentrifugation, immunodiffusion and Immunoelectrophoresis. The enzyme was a homotetramer with a molecular weight of 210,000 ± 5000. The enzyme showed homotropic cooperative interactions with tetrahydrofolate (nH = 2.8) and a hyperbolic saturation pattern with L-serine. At the lowest concentration of tetrahydrofolate used (0.2 mM), only 5% of the added folate was oxidized during preincubation and assay. ThenH value was independent of the time of preincubation. Preincubation of the enzyme with serine resulted in a partial loss of the cooperative interactions (nH =1.6) with tetrahydrofolate. The enzyme was regulated allosterically by interaction with nicotinamide nucleotides; NADH was a positive effector while NAD+ was a negative allosteric effector. The subunit interactions were retained even at the temperature optimum of 60‡C unlike in the case of the monkey liver enzyme, where these interactions were absent at higher temperatures. D-Cycloserine, a structural analogue of serine caused a sigmoid pattern of inhibition, in contrast with the observations on the monkey liver enzyme. Cibacron blue F3GA completely inhibited the enzyme and this inhibition could be reversed by tetrahydrofolate. Unlike in the monkey liver enzyme, NAD+ and NADH gave considerable protection against this inhibition. The sheep liver enzyme differs significantly in its kinetic and regulatory properties from the serine hydroxymethyltransferases isolated from other sources.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Serine Hydroxymethyltransferase; Allostery; Tetrahydrofolate
ID Code:21241
Deposited On:20 Nov 2010 13:16
Last Modified:17 May 2016 05:26

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