Nucleotidases in plants: III. Effect of metabolites on the enzyme hydrolyzing flavine adenine dinucleotide (FAD) from Phaseolus radiatus

Ravindranath, S. D. ; Appaji Rao, N. (1969) Nucleotidases in plants: III. Effect of metabolites on the enzyme hydrolyzing flavine adenine dinucleotide (FAD) from Phaseolus radiatus Archives of Biochemistry and Biophysics, 133 (1). pp. 54-59. ISSN 0003-9861

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000398...

Related URL: http://dx.doi.org/10.1016/0003-9861(69)90487-1

Abstract

The highly purified enzyme from mung bean seedlings hydrolyzing FAD at pH 9.4 and temperature 49°, functioned with an initial fast rate followed by a second slower rate. The activity was linear with enzyme concentration over a small range of concentration and was dependent on the time of incubation. Inhibition of enzyme activity with increasing concentrations of AMP was sigmoid; concentrations less than 1 × 10-6 M were without effect, concentrations between 1 × 10-6 and 8 × 10-5 M inhibited by 20% and concentrations beyond 8 × 10-5 M caused progressive inhibition. Concentrations beyond 1 × 10-3 M inhibited the activity completely. Preincubation of the enzyme with PCMB or NEM, or aging, or reversible denaturation with urea abolished the inhibitory effect of AMP at concentrations lower than 8 × 10-6 M. The aged enzyme could be reactivated by ADP.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:21229
Deposited On:20 Nov 2010 14:47
Last Modified:12 May 2011 18:00

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