The immunofluorescent detection of Entamoeba histolytica in pus using avidin-biotin system

Gandhi, B. M. ; Irshad, M. ; Acharya, S. K. ; Tandon, B. N. (1987) The immunofluorescent detection of Entamoeba histolytica in pus using avidin-biotin system Journal of Microbiological Methods, 7 (4-5). pp. 169-177. ISSN 0167-7012

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/016770...

Related URL: http://dx.doi.org/10.1016/0167-7012(87)90037-6

Abstract

Based on biotin-avidin interaction, labelled proteins were used to develop an immunofluorescence technique to detect amoebae in samples of pus. Using this technique, Entamoeba histolytica has been demonstrated in 18 of 19 pus samples aspirated from amoebic liver abscess. None of the 17 controls samples obtained from pyogenic abscess of non-amoebic origin showed the presence of E. histolytica. The test was specific, sensitive and easy to perform and is recommended for diagnosis of amoebic liver abscess.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Biotin-Avidin; Fluorescence; ALA
ID Code:209
Deposited On:17 Sep 2010 09:01
Last Modified:12 May 2011 04:18

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