Serum levels of dihydrotestosterone in male rhesus monkeys estimated by a non-chromatographic radioimmunoassay method

Puri, V. ; Puri, C. P. ; Anand Kumar, T. C (1981) Serum levels of dihydrotestosterone in male rhesus monkeys estimated by a non-chromatographic radioimmunoassay method Journal of Steroid Biochemistry, 14 (9). pp. 877-881. ISSN 0022-4731

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002247...

Related URL: http://dx.doi.org/10.1016/0022-4731(81)90236-3

Abstract

Since the antiserum used to measure dihydrotestosterone (DHT) has considerable cross-reaction with testosterone (T), the chromatographie separation of DHT from T is a pre-requisite to get precise estimates of DHT levels by radioimmunoassay (RIA) in biological fluids. To simplify this procedure a non-chromatographic method for the estimation of DHT is reported. The treatment of T with 0.5% aqueous solution of potassium permanganate (KMnO4) for 30 min, at room temperature, selectively destroys its cross-reactivity with antiserum raised against T-3(O-carboxy-methyl)-oxime-bovine serum albumin. Whereas, the thin layer Chromatographie mobility of DHT in benzene-ethyl acetate solvent system (2:1, v/v) and its cross-reaction with T-antiserum remain unaffected (70%) following treatment with KMnO4. Thus by treating the ether extracts of biological samples with KMnO4 the endogenous T is inactivated and a simple non-chromatographic method is validated for quantitating DHT by RIA. In this assay procedure the serial dilutions of pools of serum samples from adult rhesus monkeys show dose-response curves that are parallel to the DHT standard curve. The correlation coefficient of DHT estimates obtained after Chromatographie separation of DHT on sephadex LH-20 column, using cyclohexane-benzene-methanol (80:15:15, by vol.) solvent system, or following KMnO4 treatment of ethereal extracts of serum samples is 0.99. Using this method, the geometric mean serum levels of DHT. measured in 20 adult male rhesus monkeys, are significantly (P < 0.05) higher at night (5.38 nmol/l) than during the day (3.22 nmol/l).

Item Type:Article
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Keywords:(DHT); Dihydrotestosterone; 17β-hydroxy-5-androstan-3-one; Testosterone; 17β-hydroxy-4-androsten-3-one
ID Code:2082
Deposited On:08 Oct 2010 09:23
Last Modified:12 May 2011 07:37

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