Detection of genome specific monomorphic loci in Bos taurus and Bubalus bubalis with oligodeoxyribonucleotide probe

Ali, S. ; Bala, G. ; Bala, S. (1993) Detection of genome specific monomorphic loci in Bos taurus and Bubalus bubalis with oligodeoxyribonucleotide probe Animal Genetics, 24 (3). pp. 199-202. ISSN 0268-9146

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Official URL: http://www3.interscience.wiley.com/journal/1193021...

Related URL: http://dx.doi.org/10.1111/j.1365-2052.1993.tb00288.x

Abstract

A synthetic oligodeoxyribonucleotide probe (OAT36) comprising nine repeats of 5'GACA 3' and several enzymes were used to analyse cow, (Bos taurus) and buffalo (Bubalus bubalis) genomes and a number of monomorphic loci were detected in both the species. Different animals from the same species showed an almost 'similar' monomorphic hybridization pattern but animals from two separate species showed a different 'genome specific' pattern. The overall hybridization with any enzyme and probe combination was found to be unique to one species. This forms the basis of genome specific hybridization which is substantiated by our zoo-blot hybridization studies. The evolutionary aspect of these loci in the context of sequence polymorphisms is discussed.

Item Type:Article
Source:Copyright of this article belongs to International Society for Animal Genetics.
Keywords:DNA Probe; Genome Analysis; Monomorphic Loci
ID Code:20762
Deposited On:20 Nov 2010 13:36
Last Modified:11 May 2011 11:20

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