Enhancement of bacterial gene expression by insertion elements or by mutation in a CAP-cAMP binding site

Reynolds, Ann E. ; Mahadevan, Subramony ; LeGrice, Stuart F. J. ; Wright, Andrew (1986) Enhancement of bacterial gene expression by insertion elements or by mutation in a CAP-cAMP binding site Journal of Molecular Biology, 191 (1). pp. 85-95. ISSN 0022-2836

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002228...

Related URL: http://dx.doi.org/10.1016/0022-2836(86)90424-9

Abstract

The regulatory region (bglR) of the cryptic bgl operon was characterized by DNA sequence analysis and transcription mapping. Bgl--specific transcription was found to occur in both the wild-type Bgl- and mutant Bgl+ cells. However, the steady-state level of bgl RNA was much higher in the Bgl+ mutant than in the wild-type. Activation of the bgl operon by insertion sequence-mediated bglR mutations or point mutations in bglR is therefore the result of increased transcription. The ethylmethane sulfonate-induced point mutations in bglR are alterations in a single base in the cAMP binding protein (CAP) binding site, leading to a stronger binding of the CAP-cAMP complex. The IS1 and IS5-mediated bglR mutations analyzed show that the insertion sequences can activate the bgl operon by integration 78 to 125 base-pairs upstream from the transcription initiation site. The role of the insertion sequences in activation of the bgl operon is discussed.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:20728
Deposited On:20 Nov 2010 13:40
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