DNA finger printing by oligonucleotide probes specific for simple repeats

Ali, S. ; Muller, C. R. ; Epplen, J. T. (1986) DNA finger printing by oligonucleotide probes specific for simple repeats Human Genetics, 74 (3). pp. 239-243. ISSN 0340-6717

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Official URL: http://www.springerlink.com/content/p21202658h2330...

Related URL: http://dx.doi.org/10.1007/BF00282541


Interspersed simple repetitive DNA is a convenient genetic marker for analysis of restriction fragment length polymorphisms (RFLPs) because of the numbers and the frequencies of its alleles. Oligonucleotide probes specific for variations of the GATCA simple repeats have been designed and hybridized to a panel of human DNAs digested with various restriction enzymes. Numerous RFLPs were demonstrated in AluI and MboI digested DNA with "pure" GATA oligonucleotides as probes. The optimal length of the probe for RFLP analysis was 20 bases taking into account fragment lengths (1.5-7 kilobases = kb), signal to background ratio, and number of clearly evaluable RFLPs. By using different restriction enzymes individual-specific hybridization patterns ("DNA fingerprints") can be established. Hypervariable simple repeat fragments are stably inherited in a Mendelian fashion. Advantages of this method are discussed.

Item Type:Article
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ID Code:20675
Deposited On:20 Nov 2010 13:45
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