Lysis of vibrio cholerae cells: direct isolation of the outer membrane from whole cells by treatment with urea

Lohia, Anuradha ; Chatterjee, Anadi N. ; Das, Jyotirmoy (1984) Lysis of vibrio cholerae cells: direct isolation of the outer membrane from whole cells by treatment with urea Journal of General Microbiology, 130 . pp. 2027-2033. ISSN 0022-1287

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Official URL: http://mic.sgmjournals.org/cgi/content/abstract/13...

Related URL: http://dx.doi.org/10.1099/00221287-130-8-2027

Abstract

Cells of Vibrio cholerae underwent rapid autolysis when suspended in media of low osmolarity under non-growing conditions. Chaotropes like urea and guanidine. HCl which are potent protein denaturants caused complete and immediate lysis of whole cells. This unique sensitivity of V. cholerae to protein denaturants led to the development of a rapid method for the selective isolation of the outer membrane upon treatment of whole cells with urea. The composition of the outer membrane isolated from both whole cells and crude envelopes by treatment with urea was comparable with that of the outer membrane isolated by other conventional methods.

Item Type:Article
Source:Copyright of this article belongs to Society for General Microbiology.
ID Code:19475
Deposited On:22 Nov 2010 12:32
Last Modified:17 May 2016 04:01

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