Molecular cloning of a rho family gene of Entamoeba histolytica

Lohia, Anuradha ; Samuelson, John (1993) Molecular cloning of a rho family gene of Entamoeba histolytica Molecular and Biochemical Parasitology, 58 (1). pp. 177-180. ISSN 0166-6851

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/016668...

Related URL: http://dx.doi.org/10.1016/0166-6851(93)90104-6 |

Abstract

An Entamoeba histolytica gene (Eh rhol) was cloned that encodes a putative low-molecular-mass GTP-binding protein, most similar to the ras homologue rho. The Eh rhol open reading frame was 208 amino acids long and encoded a 23-kDa protein similar to Saccharomyces cerevisiae RHO1-RH04 and CDC42 and human rhoA, racl. and G25K, gene products. This similarity was greatest at the NH2 terminus of Eh rhol where two GTP-binding sites and a possible effector site were conserved. A cysteine residue at the COOH terminus of Eh rhol was followed by eight hydrophobic amino acids rather than the three hydrophobic amino acids present in other ras family proteins.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Entamoeba histolytica; Amoeba; GTP-binding Protein; ras; rho; CDC42
ID Code:19452
Deposited On:22 Nov 2010 12:34
Last Modified:22 Nov 2010 12:34

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