Identification of 9-O-acetylated sialoglycans on peripheral blood mononuclear cells in Indian visceral leishmaniasis

Bandyopadhyay, Sumi ; Chatterjee, Mitali ; Sundar, Shyam ; Mandal, Chitra (2004) Identification of 9-O-acetylated sialoglycans on peripheral blood mononuclear cells in Indian visceral leishmaniasis Glycoconjugate Journal, 20 (9). pp. 531-536. ISSN 0282-0080

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Official URL: http://www.springerlink.com/content/l171653n633086...

Related URL: http://dx.doi.org/10.1023/B:GLYC.0000043289.86611.44

Abstract

Although the existence of O-acetylated sialic acids is well known, it is only in recent years that steady refinement of analytical techniques have enabled detailed mapping of their structural diversity [1]. Fluorimetric analysis of peripheral blood mononuclear cells (PBMC) of patients with Visceral Leishmaniasis (VL) showed six fold increase in the percentage of surface 9-O-acetylated sialoglycoconjugates (9-O-AcSGs) as compared to normal human donors. Using Achatinin-H, a 9-O-acetyl sialic acid- binding lectin, an enhanced presence of 9-O-AcSGs in an α 2 → 6 linkage was demonstrated by flow cytometry; abolition of its binding by pretreatment with a recombinant 9-O-acetylesterase corroborated the presence of this glycotope. Western blotting of PBMC from VL patients indicated the presence of five O-acetylated sialoglycans corresponding to 144, 65, 56, 36 and 19 kDa as compared to 144 and 36 kDa in normal individuals. Taken together our data indicates that during active disease, there is an overexpression of 9-O-AcSGs on the surface of PBMC of VL patients, thus opening up new research avenues wherein the expression of this biomarker could be exploited to monitor the clinical status of VL patients.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Visceral Leishmaniasis; 9-O-acetylated Sialic Acids; Sialic Acids; Achatinin-H; 9-O-acetyl Sialic Acid Binding Lectin
ID Code:19374
Deposited On:22 Nov 2010 12:42
Last Modified:28 Feb 2011 09:36

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