Sarkar, Manju ; Mandal, Chitra (1985) Immobilization of antibodies on a new solid phase for use in ELISA Journal of Immunological Methods, 83 (1). pp. 55-60. ISSN 0022-1759
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002217...
Related URL: http://dx.doi.org/10.1016/0022-1759(85)90057-2
Abstract
Antibodies to myoglobin were immobilized by covalent linkage to polyester film for use in a solid-phase ELISA. The covalent linkage of antibody to this new solid phase was accomplished by partial acid hydrolysis of the film followed by periodate oxidation. About 60 ng of protein could be immobilized per cm2 of film and the binding was stable. Antimyoglobin IgM immobilized on the films was used in the ELISA technique to detect myoglobin within the range 0.25-10 ng. The assay procedure was found to be very accurate and the coefficient of variation of each concentration ranged from 0.63 to 2.1%. Furthermore the mobilized film could be re-used after dissociating the antigen antibody complexes.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | ELISA; IgM Antibody to Myoglobin; IgM-HRP Conjugate; Polyester Solid Phase |
| ID Code: | 19321 |
| Deposited On: | 23 Nov 2010 13:08 |
| Last Modified: | 28 Feb 2011 10:09 |
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