Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin

Sen, Goutam ; Mandal, Chitra ; Chowdhury, Mridula (1992) Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin Molecular and Cellular Biochemistry, 117 (2). pp. 133-138. ISSN 0300-8177

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Official URL: http://www.springerlink.com/content/k18q1783645l71...

Related URL: http://dx.doi.org/10.1007/BF00230752

Abstract

A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25°C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:AchatininH; Albumen Gland; Sialic Acid Binding Lectin
ID Code:19296
Deposited On:23 Nov 2010 13:11
Last Modified:28 Feb 2011 09:56

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