Modification of brain fructose-1, 6-bisphosphatase activity by chelators: "Induction" of 5'-AMP sensitivity

Chattoraj, Sanghita ; Lahiri Majumder, Arun (1986) Modification of brain fructose-1, 6-bisphosphatase activity by chelators: "Induction" of 5'-AMP sensitivity Biochemical and Biophysical Research Communications, 139 (2). pp. 571-580. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1016/S0006-291X(86)80029-8

Abstract

Brain fructose-1, 6-bisphosphatase (EC 3.1.3.11) from various sources are ordinarily insensitive to 5'-AMP. In addition to stimulation and conferring a "neutral" behaviour, prior treatment with histidine, EDTA or imidazole renders the brain enzyme sensitive to 5'-AMP. The histidine treated enzyme(s) bind to Blue-Sepharose CL-6B column and are specifically eluted by 5'-AMP in contrast to the untreated enzyme(s) which do not bind to the affinity column at all. The histidine effect in inducing 5'-AMP sensitivity was abolished by treatment of the native enzyme by subtilisin or by a number of divalent cations including Zn++.

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ID Code:19250
Deposited On:23 Nov 2010 13:15
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