L-Myo-inositol-1-phosphate synthase from lower plant groups: partial purification and properties of the enzyme from Euglena gracilis

Gupta, Sarmila Das ; Adhikari, Jukta ; Lahiri Majumder, Arun (1984) L-Myo-inositol-1-phosphate synthase from lower plant groups: partial purification and properties of the enzyme from Euglena gracilis Physiologia Plantarum, 61 (3). pp. 412-416. ISSN 0031-9317

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1399-...

Related URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb06348.x

Abstract

A survey for the enzyme L-myo-inositol-1-phosphate synthase (EC 5.5.1.4) has been conducted among various members of the lower plant groups, mainly algac, bryophytes and fungi; some properties of the partially purified enzyme from Euglena gracilis Z. are presented. The enzyme was detected in Chloropycean algae, Marchantiales and the Basidiomycetous fungi. The enzyme from Euglena had a pH optimum at 7.5. The Km for glucose-6-P was 2.1 mM and for NAD+ 80 μM. When assayed in the absence of added NAD+, the enzyme showed a basal activity suggesting the presence of bund NAD+ in the system. NH4Cl increased the enzyme activity two-fold, altough the enzyme was inactivated by (NH4)2SO4.

Item Type:Article
Source:Copyright of this article belongs to Scandinavian Society for Plant Physiology.
Keywords:Inositol Synthase; Myo-inositol
ID Code:19215
Deposited On:23 Nov 2010 13:18
Last Modified:06 Jun 2011 04:10

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