Crystallization and X-ray analysis of a multienzyme complex containing RUBISCO and RuBP

Hosur, M. V. ; Sainis, J. K. ; Kannan, K. K. (1993) Crystallization and X-ray analysis of a multienzyme complex containing RUBISCO and RuBP Journal of Molecular Biology, 234 (4). pp. 1274-1278. ISSN 0022-2836

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00222...

Related URL: http://dx.doi.org/10.1006/jmbi.1993.1681

Abstract

Single crystals of a multienzyme complex isolated from spinach leaves, and containing RUBISCO bound to the substrate RuBP have been grown and characterized. The crystals belong to the orthorhombic space group P 21212 with a = 173 Å, b = 134 Å and c = 112 Å, and contain two enzyme complex molecules in the unit cell. Diffraction data to 2.5 Å resolution have been collected on the synchrotron source at the photon factory in Japan. Initial structure determination has been carried out using the molecular replacement method. The RUBISCO molecule in the complex has the normal L8S8 subunit configuration, and difference electron density is clearly observed for the other component enzymes and the RuBP substrate.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Crystallization; RUBISCO; Multienzyme Complex; Synchrotron; Molecular Replacement
ID Code:19106
Deposited On:25 Nov 2010 14:19
Last Modified:03 Jun 2011 08:35

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