Purification, crystallisation and preliminary X-ray diffraction study of ribosome inactivating protein: saporin

Kumar, Mukesh ; Dattagupta, Sharmishtha ; Kannan, K. K. ; Hosur, M. V. (1999) Purification, crystallisation and preliminary X-ray diffraction study of ribosome inactivating protein: saporin Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1429 (2). pp. 506-511. ISSN 0167-4838

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01674...

Related URL: http://dx.doi.org/10.1016/S0167-4838(98)00267-2

Abstract

We report here the crystallisation and molecular replacement results on the structure determination of S-9 isoform of the ribosome inactivating protein saporin. The protein was purified to homogeneity by a simple and efficient protocol. The crystals belong to the space group I41 with a=b=91.47 Å, c=150.66 Å and contain two molecules in the asymmetric unit.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Ribosome Inactivating Protein; Crystal; X-ray; Structure
ID Code:19097
Deposited On:25 Nov 2010 14:19
Last Modified:03 Jun 2011 08:30

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