Effect of environmental pollutants on the c-reactive protein of a freshwater major carp, Catla catla

Paul, Indrani ; Mandal, Chhabinath ; Mandal, Chitra (1998) Effect of environmental pollutants on the c-reactive protein of a freshwater major carp, Catla catla Developmental & Comparative Immunology, 22 (5-6). pp. 519-532. ISSN 0145-305X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01453...

Related URL: http://dx.doi.org/10.1016/S0145-305X(98)00031-7

Abstract

C-reactive proteins (CRP) have been affinity purified to electrophoretic homogeneity from the sera of major carp, Catla catla before and after exposure to environmental pollutants. Exposure to these pollutants elevate the levels of circulating CRPs to 2.8-3.5 times the normal values. Kinetic studies of metal intoxication indicate that a unique molecular variant of CRP is present in the serum at the peak level of acute phase induction, and this variant coexists with normal CRPs. Carbohydrate analysis and lectin binding reveals that these CRPs are glycoproteins differing significantly in total carbohydrate contents. Their electrophoretic mobilities in native gel are different but become identical on desialylation and deglycosylation implying that the molecular variants vary in the glycan parts. All these forms of CRP contain two non-identical subunits of Mr 22 and 29 kDa. Examination of their immunological crossreactivity demonstrate their similarity in overall molecular topology but their differences in the quantitative extent of binding are reflected.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:C-reactive protein (CRP); Fish (Catla catla); Aquatic Environmental Pollution; Heavy Metals (Mercury; Cadmium); Molecular Variants
ID Code:18975
Deposited On:25 Nov 2010 14:40
Last Modified:28 Feb 2011 09:52

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