Gangwani, Laxman ; Tamot, Bhaju K. ; Khurana, Jitendra P. ; Maheshwari, Satish C. (1991) Identification of 3',5'-cyclic AMP in axenic cultures of Lemna paucicostata by high-performance liquid chromatography Biochemical and Biophysical Research Communications, 178 (3). pp. 1113-1119. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(91)91007-Y
Abstract
Nucleotides were purified from the extract of aseptically grown Lemna paucicostata 6746 by low-pressure column chromatography and thin-layer chromatography. In this partially purified sample, a compound has been identified, using a highly sensitive HPLC-fluorometric detection method, with chromatographic properties and substrate specificity for cyclic nucleotide phosphodiesterase similar to authentic 3',5'-cyclic AMP. The level of cAMP has been estimated to be 70-80 pmol/g fresh wt.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 18706 |
| Deposited On: | 17 Nov 2010 12:33 |
| Last Modified: | 06 Jun 2011 11:36 |
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