Plantlets from mesophyll protoplasts of Solanum xanthocarpum

Saxena, Praveen K. ; Gill, R. ; Rashid, A. ; Maheshwari, S. C. (1982) Plantlets from mesophyll protoplasts of Solanum xanthocarpum Plant Cell Reports, 1 (5). pp. 219-220. ISSN 0721-7714

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Young leaves of Solanum xanthocarpum from axenic shoot cultures released viable protoplasts when treated with appropriate enzymes. The protoplasts on culture in modified Murashige and Skoog (1962) medium supplemented with 2,4-dichlorophenoxy-acetic acid (0.5 mg/l), naphtha leneacetic acid (1 mg/l), kinetin (1 mg/l) and organic nutrients of KM (Kao and Michayluk 1975) regenerated to form callus tissue as a result of repeated divisions. Protoplast-derived calli differentiated into shoots on MS medium enriched with kinetin (0.5 mg/l) and rooting could be initiated by transferring the shoot-buds to basal medium.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
ID Code:18673
Deposited On:17 Nov 2010 12:36
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