Androgenic response from cultured anthers of a leguminous tree, Cassia siamea Lam

Gharyal, Paramjit K. ; Rashid, A. ; Maheshwari, S. C. (1983) Androgenic response from cultured anthers of a leguminous tree, Cassia siamea Lam Protoplasma, 118 (1). pp. 91-93. ISSN 0033-183X

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Official URL: http://www.springerlink.com/content/ku51n528373504...

Related URL: http://dx.doi.org/10.1007/BF01284750

Abstract

Anthers of Cassia siamea, on culture on B5 medium supplemented with coconut milk (15%, v/v), 2,4-D (2 mg/l), and kinetin (0.5 mg/l), split open after one to two weeks of inoculation and eject a callus mass. Microscopic examination of the anthers cultured at the late uninucleate or early bi-celled stages, after 7-14 days of culture, revealed many multicellular structures at various stages of development, thus indicating the pollen origin of callus. Callus cells also showed the haploid chromosome number (n=14).

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Androgenesis; Callus; Cassia siamea; Pollen Embryoids
ID Code:18586
Deposited On:17 Nov 2010 12:46
Last Modified:17 May 2016 03:17

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