Regeneration of plants from mesophyll protoplasts of Nicotiana plumbaginifolia Viv

Gill, Ravinder ; Rashid, A. ; Maheshwari, S. C. (1978) Regeneration of plants from mesophyll protoplasts of Nicotiana plumbaginifolia Viv Protoplasma, 96 (3-4). pp. 375-379. ISSN 0033-183X

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Official URL: http://www.springerlink.com/content/n954t466436181...

Related URL: http://dx.doi.org/10.1007/BF01287697

Abstract

Protoplasts of Nicotiana plumbaginifolia were isolated by a one step enzymatic method. They were cultured in Ohyama and Nitsch's medium supplemented with 2,4-D (1.0 mg/l), benzylaminopurine (1.0 mg/l) and 14% sucrose. Cell divisions were initiated after 5 days and within 3 weeks colonies were discernible without the microscope. After transfer to Murashige and Skoog's medium containing IAA and kinetin the colonies differentiated into plantlets.

Item Type:Article
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ID Code:18582
Deposited On:17 Nov 2010 09:33
Last Modified:17 May 2016 03:17

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