Isolation and culture of protoplasts of Capsicum annuum L. and their regeneration into plants flowering in vitro

Saxena, P. K. ; Gill, R. ; Rashid, A. ; Maheshwari, S. C. (1981) Isolation and culture of protoplasts of Capsicum annuum L. and their regeneration into plants flowering in vitro Protoplasma, 108 (3-4). pp. 357-360. ISSN 0033-183X

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Official URL: http://www.springerlink.com/content/bnq666h724j353...

Related URL: http://dx.doi.org/10.1007/BF02224429

Abstract

Axenic shoot cultures of Capsicum annuum cv. California Wonder were used as the source for isolation of protoplasts from mesophyll cells. Protoplasts underwent sustained mitotic activity and proliferated to form callus masses on NT or DPD medium enriched with 2,4-D, NAA and BAP each at 1 mg/l level. The callus could be differentiated into whole plants on the differentiation media and plants floweredin vitro under long day conditions.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Capsicum annuum; Protoplast Regeneration
ID Code:18579
Deposited On:17 Nov 2010 09:32
Last Modified:17 May 2016 03:17

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