Plantlet formation from isolated protoplasts of Solanum melongena L

Abstract

Mesophyll protoplasts were isolated from axenic shoot cultures of Solanum melongena by the one-step enzymatic method. Of the different media employed for the culture of protoplasts, a medium modified from Kao and Michayluk (1975) supported sustained mitotic cycles most effectively. Organogenesis from protoplast-derived callus was achieved on transfer to Murashige and Skoog'S (1962) medium supplemented with an appropriate auxin and a cytokinin.