Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development

Iyer, Srividhya V. ; Ramakrishnan, Mukund ; Kasbekar, Durgadas P. (2009) Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development Journal of Genetics, 88 (1). pp. 33-39. ISSN 0022-1333

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Official URL: http://www.ias.ac.in/jgenet/Vol88No1/33.pdf

Related URL: http://dx.doi.org/10.1007/s12041-009-0005-2

Abstract

The Neurospora crassa fmf-1 mutation exerts an unusual 'perithecium-dominant' developmental arrest; fmf-1 × fmf-1+ cross becomes arrested in perithecial development regardless of whether the mutant participates in the cross as the male or female parent. We localized fmf-1 to the LG IL genome segment between the centromere-proximal breakpoint of the chromosome segment duplication Dp(IL)39311 and the centromere. By mapping crossovers with respect to RFLP markers in this region we further localized fmf-1 to an approximately 34-kb-genome segment. Partial sequencing of this segment revealed a point mutation in the gene NCU 09387.1, a homologue of the Schizosaccharomyces pombe ste11+ regulator of sexual development. The fmf-1 mutation did not complement a NCU 09387.1 deletion mutation, and transformation with wild-type NCU 09387.1 complemented fmf-1. S. pombe Ste11 protein (Ste11p) is a transcription factor required for sexual differentiation and for the expression of genes required for mating pheromone signalling in matP and matM cells. If FMF-1 also plays a corresponding role in mating pheromone signalling in Neurospora, then protoperithecia in an fmf-1 × fmf-1+ cross would be unable to either send or receive sexual differentiation signals and thus become arrested in development.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Perithecial Development; Chromosome Segment Duplications; Mating Pheromones; Yeast; Neurospora
ID Code:18401
Deposited On:17 Nov 2010 09:16
Last Modified:17 May 2016 03:07

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