Plant regeneration from protoplasts of Capsicum annuum L. cv. California wonder

Prakash, A. H. ; Sankara Rao, Kolluri ; Udaya Kumar, M. (1997) Plant regeneration from protoplasts of Capsicum annuum L. cv. California wonder Journal of Biosciences, 22 (3). pp. 339-344. ISSN 0250-5991

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Plant regeneration from mesophyll protoplasts of pepper,Capsicum annuum L. cv. California Wonder has been demonstrated via shoot organogenesis. Protoplasts isolated from fully expanded leaves of 3-week-old axenic shoots when cultured in TM medium supplemented with 1 mg l -1 NAA, 1 mg l -1 2,4-D, 0 5 mg l -1 BAP (CM 1) resulted in divisions with a frequency ranging from 20-25 %. Antioxidant ascorbic acid and polyvinylpyrrolidone (PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed in TM medium containing 2 mg l -1 NAA and 0.5 mg l -1 BAP (CM II) and MS gelled medium containing 2 mg 1 -1 NAA and 0 5 mg 1 -1 BAP (CM III), respectively. Regeneration of plantlets was possible via caulogenesis. Microshoots, 2-5 percallus appeared on MS gelled medium enriched with 0.5 mg l -1 IAA, 2mg l -1 GA and l0mg l -1 BAP (CM IVc). Rooting of microshoots was obtained on half strength gelled medium containing 1 mg l -1 NAA and 0.5mg l -1 BAP. Protoplasts isolated from cotyledons failed to divide and degenerated eventually.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Leaf Protoplasts; Capsicum annuum L. cv California Wonder; Regeneration; Organogenesis
ID Code:18207
Deposited On:17 Nov 2010 12:59
Last Modified:17 May 2016 02:57

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