Effects of some inhibitors and carbon sources on acetylene reduction and hydrogen production of isolated heterocysts of Anabaena sp. (strain CA)

Kumar, Dhruv ; Kumar, Har Darshan (1990) Effects of some inhibitors and carbon sources on acetylene reduction and hydrogen production of isolated heterocysts of Anabaena sp. (strain CA) European Journal of Phycology, 25 (4). pp. 375-379. ISSN 0967-0262

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Related URL: http://dx.doi.org/10.1080/00071619000650421

Abstract

Metabolically active heterocysts isolated from wild-type Anabaena sp. strain CA showed high rates of light dependent acetylene reduction and H2 evolution. Fructose and erythrose significantly stimulated nitrogenase activity but not H2 evolution. DCMU and cyanide were not effective. DBMIB significantly inhibited both nitrogenase and nitrogenase-catalysed H2 evolution. This inhibition was overcome by a catalytic amount of TMPD. These data suggest that in the isolated heterocysts all electrons, irrespective of source, must pass through the plastoquinone pool before reducing ferredoxin, which in turn can reduce dinitrogen to ammonia.

Item Type:Article
Source:Copyright of this article belongs to Taylor and Francis Ltd.
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Deposited On:17 Nov 2010 13:08
Last Modified:17 May 2016 02:52

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