Protection of nitrogenase levels in dark-incubated cultures of Anabaena sp. strain CA by various carbon sources, and restoration of nitrogenase activity by oxygen

Kumar, Dhruv ; Kumar, Har Darshan (1990) Protection of nitrogenase levels in dark-incubated cultures of Anabaena sp. strain CA by various carbon sources, and restoration of nitrogenase activity by oxygen European Journal of Phycology, 25 (3). pp. 251-256. ISSN 0967-0262

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Related URL: http://dx.doi.org/10.1080/00071619000650241

Abstract

Photoautotrophically grown, nitrogen-fixing cultures of Anabaena sp. strain CA lost nitrogenase activity completely after 4 h of incubation in the dark. The original level of nitrogenase activity was restored within 3 h of re-illumination and was apparently dependent on de novo protein synthesis. Several organic carbon sources protected nitrogenase activity. The heterocysts isolated from photoautotrophically grown cultures incubated in the dark (35 min) showed negligible nitrogenase activity. When these heterocysts were exposed to oxygen, glucose or fructose during isolation, normal activity was observed only with O2. Oxygen also enhanced the rate of initial H2 evolution from isolated heterocysts.

Item Type:Article
Source:Copyright of this article belongs to Taylor and Francis Ltd.
ID Code:18065
Deposited On:17 Nov 2010 13:14
Last Modified:17 May 2016 02:50

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