Optical detection of antibody using silica-silver core-shell particles

Kalele, S. A. ; Ashtaputre, S. S. ; Hebalkar, N. Y. ; Gosavi, S. W. ; Deobagkar, D. N. ; Deobagkar, D. D. ; Kulkarni, S. K. (2005) Optical detection of antibody using silica-silver core-shell particles Chemical Physics Letters, 404 (1-3). pp. 136-141. ISSN 0009-2614

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00092...

Related URL: http://dx.doi.org/10.1016/j.cplett.2005.01.064


Nearly monodispersed spherical particles of silica were synthesized and coated with thin layer of silver nanoparticles. Silver coated silica particles, forming core-shell particles exhibited a strong surface plasmon resonance peak at 453 nm. A very small amount (20 μg) of rabbit immunoglobulin in core-shell particle solution results in to a marked shift in surface plasmon resonance. Addition of 20 μg quantity of goat anti rabbit antibodies results in to a red shift of surface plasmon resonance to 494 nm. This demonstrates that silver coated silica particles are sensitive probes for rapid antibody-anti antibody kind of interaction investigations. Fourier transform infra red spectroscopy and scanning electron microscopy have been used to interpret the optical extinction spectroscopy results.

Item Type:Article
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Deposited On:17 Nov 2010 13:29
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