pH, temperature, humidity and the dynamic force-area curve of dipalmitoyl lecithin

Colacicco, Giuseppe ; Basu, M. K. ; Scarpelli, Emile M. (1976) pH, temperature, humidity and the dynamic force-area curve of dipalmitoyl lecithin Respiration Physiology, 27 (2). pp. 169-186. ISSN 0034-5687

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Both high pH at 25°C and humidity at 37°C prevent DPL films from attaining zero surface tension, whereas humidity at 25°C and high pH at 37°C do not. At 37°C DPL lowered surface tension to zero when spread from organic solvent or when adsorbed from aqueous 0.15M Nad in the surface balance in which the surface film was exposed to the room air (dry film). Upon saturation of the atmosphere with water vapor in equilibrium with the aqueous phase at 37°C in a closed chamber, DPL lost the ability to produce zero surface tension, and the V of the DPL film increased from zero to 22 dyne/cm. Addition of DPL in chloroform to distilled water before dispersion by sonication did not prevent the effect of the humidity. However, when the chloroform solution of DPL was added to 0.15 M Nad before sonication, the adsorbed film produced immediately a stable V of zero in a saturated atmosphere, 37°C. In the absence of chloroform, with DPL adsorbed from either distilled water or 0.15M Nad, the effect of humidity was reversed either by removing the chamber and returning the wet film to room air or by introducing small quantities of dispersing agents such as cholesteryl palmitate. However, whereas the effect of humidifying the air was reversible indefinitely, the effect of cholesteryl palmitate (zero surface tension, wet or dry film) was irreversible. This means that there are substances or conditions that can assist DPL films in maintaining zero surface tension when such films are exposed to humidity-saturated air at 37°C.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Cholesteryl Palmitate; pH and Temperature; Dipalmitoyl Lecithin; Spread and Adsorbed Film; Dry and Wet Film; Zero Surface Tension; Force-area Curves
ID Code:1732
Deposited On:05 Oct 2010 12:03
Last Modified:14 May 2011 10:23

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