Krishnan, V. ; Narisimhan, T. R. ; Safe, S. (1992) Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells Chemosphere, 25 (7-10). pp. 947-950. ISSN 0045-6535
Full text not available from this repository.
Official URL: http://linkinghub.elsevier.com/retrieve/pii/004565...
Related URL: http://dx.doi.org/10.1016/0045-6535(92)90089-A
Abstract
The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-DTM kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 17294 |
| Deposited On: | 16 Nov 2010 08:02 |
| Last Modified: | 04 Jun 2011 06:40 |
Repository Staff Only: item control page
