Effects of prostaglandins E₂ and F_2α on lecithin biosynthesis by cultured lung cells

Abstract

Transformed cells from human lung carcinoma (Line A549), resembling type II pneumocytes, were cultured in monolayer at 37°C and incubated for five hours with ³H-choline and ¹⁴C-palmitate in the presence of various concentrations of prostaglandins (PGs) E₂ and F_2α. In the control (no PG) the level of % palmitate incorporation was 13.5 × as high as that of choline, after taking isotope dilution into account. Between the concentrations studied, 0.1 and 10 μM, both prostaglandins stimulated markedly the incorporation of both precursors, though choline up to 3 × better than palmitate. This was indicated by a change in the palmitate/choline incorporation ratio from 13.5 to as low as 4.2. At the lowest PG concentration, 0.1 μM, PGE₂ was much more effective than PGF_2α in stimulating the incorporation of both precursors.