Effect of serum albumin on dynamic force-area curve of dipalmitoyl lecithin

Colacicco, Giuseppe ; Basu, M. K. (1978) Effect of serum albumin on dynamic force-area curve of dipalmitoyl lecithin Respiration Physiology, 32 (3). pp. 265-279. ISSN 0034-5687

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/003456...

Related URL: http://dx.doi.org/10.1016/0034-5687(78)90115-9


In line with previous findings at 25°C, solutions of serum albumin in the subphase stabilized the surface activity of DPL spread films at 25°C as well as 37°C. In contrast, films adsorbed from mixtures of DPL and albumin exhibited a marked inhibitory action of the albumin on DPL activity. The inhibitory effect increased with the relative protein concentration but, with albumin/DPL ratios smaller than 2, the DPL activity was regained gradually with cycling. With larger albumin/DPL ratios the adsorbed films retained the albumin character permanently. The negative effect of albumin was counteracted by higher temperatures (37°C vs 25°C) and modest cholesterol concentrations; with greater cholesterol concentrations the known inhibitory effect of cholesterol prevailed. The inhibitory effect of albumin was potentiated by humidity; saturation of the atmosphere with water vapor at 37°C abolished the DPL character of DPL-RSA mixtures and prevented its return (zero surface tension) upon reversal of the atmosphere from saturated water vapor to dry air. The data are important in the interpretation of the surface activity of pulmonary washings and other pulmonary extracts.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Alveolar Surface Tension; Pulmonary Surfactant; Dynamic Force-area Curves; Zero Surface Tension; Dipalmitoyl Lecithin
ID Code:1726
Deposited On:05 Oct 2010 12:04
Last Modified:14 May 2011 10:18

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