Citric acid production by Aspergillus niger immobilized on cellulose microfibrils: influence of morphology and fermenter conditions on productivity

Sankpal, N. V. ; Joshi, A. P. ; Kulkarni, B. D. (2001) Citric acid production by Aspergillus niger immobilized on cellulose microfibrils: influence of morphology and fermenter conditions on productivity Process Biochemistry, 36 (11). pp. 1129-1139. ISSN 0032-9592

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00329...

Related URL: http://dx.doi.org/10.1016/S0032-9592(01)00155-8

Abstract

Continuous and batch production of citric acid from sucrose has been investigated using Aspergillus niger NCIM 588. Mycelia of A. niger grown on cellulose microfibril forms a uniform and thin mycelial proliferation under controlled conditions of cultivation rich in oxygen. In the fed batch mode using a recycle reactor, the DO of the system was maintained at 20 mg l-1 using oxygen enriched air. This improved volumetric productivity to 1.85 g l-1 h-1 of citric acid, representing an increase of at least 15-fold over results obtained simultaneously using shake-flasks and 1.6-fold over a conventional aerated batch reactor. It was possible to substitute sucrose with sugarcane juice as a carbon source in a fed batch recycle system. An overall specific production rate of citric acid of 0.147 and 0.208 g g-1 h-1 was achieved using cane juice and sucrose, respectively. In continuous fermentation, a medium containing 50 g l-1 of sucrose was allowed to drip through the fabric support at a residence time of 20 h. As a result of interface interaction, a citric acid volumetric productivity of 2.08 g l-1 h-1 was achieved for 26 days without any significant loss of productivity.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Aspergillus niger; Citric Acid; Immobilization; Cellulose Microfibrils; Morphology; Batch and Continuous Fermentation
ID Code:17222
Deposited On:16 Nov 2010 08:11
Last Modified:06 Jun 2011 09:18

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