Evidence that there are only two tRNAPhe genes in Escherichia coli

Pittard, J. ; Praszkier, J. ; Certoma, A. ; Eggertsson, G. ; Gowrishankar, J. ; Narasaiah, G. ; Whipp, M. J. (1990) Evidence that there are only two tRNAPhe genes in Escherichia coli Journal of Bacteriology, 172 (10). pp. 6077-6083. ISSN 0021-9193

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Official URL: http://jb.asm.org/cgi/content/abstract/172/10/6077

Abstract

pheV, one of the genes that code for tRNAPhe, was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate mutase P-prephenate dehydratase, the first enzyme in the terminal pathway of phenylalanine biosynthesis, was derepressed. Similar derepression of pheA has been reported in pheR mutants of E. coli K-12 (J. Gowrishankar and J. Pittard, J. Bacteriol. 150:1130-1137, 1982). Attempts to introduce a pheR mutation into the Δ pheV strain failed under circumstances suggesting that this combination of mutations is lethal. Southern blot analysis of pheV+ and Δ pheV strains indicated that there are only two tRNAPhe genes in E. coli. It is recommended that the names pheU and pheV be retained for these genes.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:16968
Deposited On:16 Nov 2010 13:13
Last Modified:17 May 2016 01:40

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